Special Stain: Masson’s Trichrome

Todayโ€™s special stain is ๐Œ๐š๐ฌ๐ฌ๐จ๐งโ€™๐ฌ ๐ญ๐ซ๐ข๐œ๐ก๐ซ๐จ๐ฆ๐ž!

๐–๐ก๐š๐ญ ๐ข๐ฌ ๐š ๐Œ๐š๐ฌ๐ฌ๐จ๐งโ€™๐ฌ ๐ญ๐ซ๐ข๐œ๐ก๐ซ๐จ๐ฆ๐ž ๐ฌ๐ญ๐š๐ข๐ง?
Trichrome stains are primarily used to identify ๐œ๐จ๐ฅ๐ฅ๐š๐ ๐ž๐ง (a structural protein), bone or ๐Ÿ๐ข๐›๐ซ๐ข๐ง (the protein that forms blood clots). In general, collagen and bone are highlighted blue-green, while fibrin is highlighted in red.

This image is a case of ๐ž๐ง๐๐จ๐œ๐š๐ซ๐๐ข๐š๐ฅ ๐Ÿ๐ข๐›๐ซ๐จ๐ฌ๐ข๐ฌ in the heart of a cat. In this condition, the ๐ž๐ง๐๐จ๐œ๐š๐ซ๐๐ข๐ฎ๐ฆ (inner lining of the heart) becomes severely thickened with scar tissue. Scar tissue is primarily made up of collagen, which is why this heart is lined with bright blue on a trichrome stain!

Photo ยฉ Noahโ€™s Arkive contributor Rech licensed under CC BY-SA 4.0.

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